Biology by Bradford · IB Biology SL + HL · A1.2

Four letters.
Two strands.
Everything alive.

A nucleotide is three parts stuck together. Chain them up, pair them off, and you have a molecule that can copy itself: and store more information than any language ever written.

01 · A1.2.1–A1.2.5 · The essentials

Three parts,
one monomer.

Nucleotide

Every nucleotide is a phosphate group, a pentose sugar and a nitrogenous base. Draw them as a circle, a pentagon and a rectangle.

Backbone

Condensation reactions join the sugar of one nucleotide to the phosphate of the next, making a continuous covalently bonded sugar–phosphate backbone. Strong, and the same all the way along.

Genetic material

DNA is the genetic material of all living organisms. Some viruses use RNA instead: but viruses are not considered to be living.

02 · A1.2.2–A1.2.6 · Structure

Take the
molecule apart.

Two antiparallel strands, three base pairs. Tap a component to isolate it: this is the diagram convention the IB expects you to draw.

A T G C T A P S P S P S P S P S P S 5′ end 3′ end 3′ end 5′ end
The convention

Circle = phosphate, pentagon = pentose sugar, rectangle = nitrogenous base. Draw the two strands antiparallel; you are not expected to draw the helical twist.

03 · A1.2.7 · DNA vs RNA

One oxygen
apart.

Three differences, and you should be able to sketch the first one from memory.

O 1′ 2′ 3′ 4′ base OH H 5′ P deoxyribose DNA · H at carbon 2′ O 1′ 2′ 3′ 4′ base OH OH 5′ P ribose RNA · OH at carbon 2′
Carbon 2′ carries H in deoxyribose and OH in ribose · that single oxygen is the whole difference in the sugar
Feature
DNA
RNA
Pentose sugar
deoxyribose
ribose
Bases
A, C, G, T
A, C, G, U
Strands
double-stranded
single-stranded
Role
master copy of the instructions
working copy, used to build proteins

Purines (two rings): adenine, guanine · Pyrimidines (one ring): cytosine, thymine, uracil

04 · A1.2.8 · Complementary base pairing

Build a strand.
The rest
writes itself.

Tap bases to build a template strand. Because pairing is fixed, the other DNA strand and the mRNA transcript follow automatically: which is exactly why genetic information can be copied at all.

DNA template strand3′5′
Complementary DNA strand5′3′
mRNA transcript5′3′
Complementarity

Adenine pairs with thymine, cytosine pairs with guanine, and both pairs are held by hydrogen bonds. Add or remove bases and watch every other row update itself.

05 · A1.2.9–A1.2.10 · Information

Why four letters
are enough.

Limitless storage

A DNA molecule can be any length and carry any sequence of bases. A stretch of just 10 bases already has 410 ≈ a million possible sequences: and it is stored with extraordinary economy.

One shared code

The same base sequences specify the same amino acids in bacteria, bananas and blue whales. A code this arbitrary being universal is powerful evidence that all life shares a common ancestor.

06 · A1.2.11–A1.2.12 · Directionality & stabilityHL

Every strand
has a direction.

5′ → 3′

The carbons of the pentose are numbered. The phosphate sits on carbon 5′ and a free OH on carbon 3′, so each new nucleotide can only be added to the 3′ end. Replication, transcription and translation all run 5′ → 3′.

Antiparallel

The two DNA strands run in opposite directions: one 5′ → 3′, the other 3′ → 5′. This is why the two strands of a replication fork are handled differently.

Constant width

A two-ring purine always pairs with a one-ring pyrimidine, so every base pair is the same length and the helix keeps the same three-dimensional shape whatever the sequence.

purine · two rings pyrimidine · one ring A T 2 H-bonds G C 3 H-bonds same width: both rows
A–T is held by two hydrogen bonds, C–G by three · a purine plus a pyrimidine spans the same distance either way
07 · A1.2.13 · NucleosomesHL

Two metres,
one nucleus.

DNA is far too long to sit loose in a nucleus. It is wound onto protein spools. Tap each component.

linker DNA to the next nucleosome 8 histones + ~147 bp of DNA H1
Nucleosome

A length of DNA wrapped around a core of eight histone proteins, with one additional histone holding it in place where the DNA enters and leaves. Repeat this along the molecule and you get the "beads on a string" arrangement.

08 · A1.2.14–A1.2.15 · The evidenceHL

How we
know.

Two classic results. One proved DNA was the genetic material; the other destroyed the leading hypothesis about its structure. Tap through the experiment.

1 · infect 2 · blend 3 · spin ³⁵S protein labelled E. coli ³⁵S stays in the liquid: the empty coats never got in ³²P DNA labelled E. coli ³²P is in the pellet: the DNA went into the cells radioactivity inside the cells came from the DNA, not the protein
Protein contains sulfur but no phosphorus · DNA contains phosphorus but no sulfur · pink marks where the radioactivity ended up
Hershey–Chase, 1952

Bacteriophages are made of only two things: protein and DNA. If you can label each one separately and see which enters the bacterium, you have your answer.

Chargaff's
numbers.

Percentages of each base measured in different organisms. Read down the columns before you read across.

Source of DNA
% A
% T
% G
% C
Human
30.9
29.4
19.9
19.8
Yeast
31.3
32.9
18.7
17.1
Herring
27.8
27.5
22.2
22.6
E. coli
24.7
23.6
26.0
25.7
Within a species

%A ≈ %T and %G ≈ %C, every time. Chargaff had no structural explanation for this: but it is exactly what you would expect if A always pairs with T and G always pairs with C.

Between species

The A+T to G+C ratio differs from organism to organism. That killed the tetranucleotide hypothesis, which claimed DNA was a monotonous repeat of the four bases in equal amounts: if it were, every row here would read 25, 25, 25, 25.

Nature of science: no amount of data can prove a hypothesis, but a single set of contrary measurements can falsify one

09 · The bigger picture

Where this
fits.

Tap a concept to light up how it connects.

polymerises to polymerises to held by wound into translated into copies the code Nucleotide DNA RNA Complementarybase pairing Nucleosome Protein
Overview

One monomer builds both nucleic acids. The pairing rule that holds the double helix together is the same rule that lets the information be copied: structure and function are the same fact seen twice.

Tap any concept to trace its connections · tap the background to reset

10 · Your turn

Fill in
the gaps.

Drag each term into the gap it belongs in. Two terms are traps.

Placed: 0 / 0
A nucleotide is a base, a phosphate and adrop
The pentose sugar found in DNA isdrop
Nucleotides are joined into a polymer bydrop
In DNA, adenine always pairs withdrop
RNA replaces thymine with the basedrop
The two strands run in opposite directions: they aredrop
The bases of the two strands are joined bydrop
HL · A nucleosome has a core of this many histones:drop
11 · Check yourself

IB-style
multiple choice.

Single best answer, Paper 1 style. HL-only questions are marked.

Score: 0 / 0