Biology by Bradford · IB Biology HL · D3.2.16–D3.2.21

9 : 3 : 3 : 1
and the
test cross.

Two genes, four gametes, sixteen boxes. Learn to read a ratio backwards: and the cross that exposes a hidden genotype.

01 · The three things

Two genes,
followed at once.

Four gametes

A double heterozygote RrTt makes four gamete types, RT, Rt, rT, rt, because unlinked genes assort independently in metaphase I.

9 : 3 : 3 : 1

Cross two double heterozygotes and the F2 phenotypes fall out as 9 : 3 : 3 : 1. Nine boxes show both dominant traits; one shows both recessive.

1 : 1 : 1 : 1

Cross anything with a homozygous recessive and you have a test cross. The offspring read out the unknown parent's gametes directly.

ALIGNMENT 1 R r T t RT rt ALIGNMENT 2 R r t T Rt rT Both alignments are equally likely: so all four gamete types are made equally often.
Random orientation of the two homologous pairs at metaphase I · each homologue drawn as a single bar for clarity
02 · D3.2.17 · Punnett grid

RrTt × RrTt.
Sixteen boxes.

Seed shape: round (R) is dominant to wrinkled (r). Plant height: tall (T) is dominant to dwarf (t). Tap a phenotype class to light up its boxes.

9 R_T_ round, tall 3 R_tt round, dwarf 3 rrT_ wrinkled, tall 1 rrtt wrinkled, dwarf
The four F2 phenotype classes · the colours match the boxes in the grid below
RrTt× RrTt RT Rt rT rt RT Rt rT rt RRTT RRTt RrTT RrTt RRTt RRtt RrTt Rrtt RrTT RrTt rrTT rrTt RrTt Rrtt rrTt rrtt
The grid

Each parent makes four gamete types, so the grid is 4 × 4 = 16 equally likely combinations. Tap a class to see which boxes produce it.

03 · D3.2.20 · The test cross

Cross it with
a double recessive.

A round, tall plant could be four different genotypes. Cross it with rrtt and the offspring give it away: because the recessive parent contributes nothing but rt.

rrtt TESTER r r t t rt one gamete type only
Homozygous recessive at both loci · it can only ever donate r and y
_ _ _ _Unknown parent
×
rrttWrinkled dwarf tester
Pick a genotype

Choose one of the four possible genotypes for the round, tall parent: or hit “Mystery plant” and work backwards from the offspring.

04 · Worked solutions

Solve it
the same way
every time.

Five steps, then two crosses solved in full. Try each one on paper first: then open the solution.

Tap a step

The method is identical whether the question asks for 9:3:3:1 or for an unknown genotype. Only the second parent changes.

Problem 1 · predict the ratio

In a tomato variety, tall stems (T) are dominant to dwarf (t), and hairy stems (H) are dominant to smooth (h). The two genes are unlinked. Two plants that are heterozygous for both genes are crossed. Determine the expected phenotypic ratio of the offspring.

Step 1 · alleles

Tall T > dwarf t; hairy H > smooth h.

Step 2 · parents

Heterozygous for both means TtHh × TtHh. Alleles are paired gene by gene: TtHh, never THth.

Step 3 · gametes

Each parent gives four types: TH, Th, tH, th. Take one allele of each gene, in every combination.

Step 4 · grid

4 × 4 = 16 equally likely boxes.

TH
Th
tH
th
TH
TTHH
TTHh
TtHH
TtHh
Th
TTHh
TThh
TtHh
Tthh
tH
TtHH
TtHh
ttHH
ttHh
th
TtHh
Tthh
ttHh
tthh
Step 5 · count phenotypes

Group the boxes by appearance: 9 have at least one T and one H; 3 are T_hh; 3 are ttH_; 1 is tthh.

9 tall hairy : 3 tall smooth : 3 dwarf hairy : 1 dwarf smooth
Problem 2 · find the genotype

In a breed of dog, black coat (B) is dominant to brown (b) and short hair (S) is dominant to long (s). A black, short-haired dog is test crossed with a brown, long-haired dog. Across several litters the puppies are: 12 black short-haired, 14 black long-haired, 11 brown short-haired, 13 brown long-haired. Deduce the genotype of the black, short-haired parent.

Step 1 · the tester

Brown and long-haired is the double recessive, bbss, so it can only donate bs. Every dominant trait in a puppy must have come from the other parent: so the puppies are a direct readout of that parent's gametes.

Step 2 · read the coat colour

Black 12 + 14 = 26, brown 11 + 13 = 24. Roughly 1 : 1, so coat colour segregated and the unknown parent is heterozygous: Bb.

Step 3 · read the hair length

Short 12 + 11 = 23, long 14 + 13 = 27. Again roughly 1 : 1, so hair length segregated too: Ss.

Step 4 · check it

BbSs × bbss gives four gametes (BS, Bs, bS, bs) against bs only → 1 BbSs (black short) : 1 Bbss (black long) : 1 bbSs (brown short) : 1 bbss (brown long). Four phenotypes in equal numbers, 1 : 1 : 1 : 1, which is what the data show.

Watch for

The four classes appearing at all is the giveaway. Had the parent been BBSs, no brown puppies could have appeared; had it been BBSS, every puppy would have been black and short-haired.

The black, short-haired parent is BbSs

Note: ratios are probabilities · real litters and real F2 populations scatter around them

05 · The bigger picture

Where this
fits.

Tap a concept to light up how it connects.

shuffles alleles underpins predicts extended by reveals linkage χ² deviation Meiosis I Independentassortment Dihybrid cross 9 : 3 : 3 : 1 Test cross Linked genes
Overview

Independent assortment in meiosis I is what makes the 9:3:3:1 ratio possible; when a cross fails to give it, the genes were probably never independent in the first place.

Tap any concept to trace its connections · tap the background to reset

06 · Your turn

Fill in
the gaps.

Drag each term into the gap it belongs in. Two terms are traps.

Placed: 0 / 0
A plant of genotype RrTt makes this many gamete types:drop
Two double heterozygotes give an F2 phenotype ratio ofdrop
In a test cross, the second parent is alwaysdrop
RrTt × rrtt gives a phenotype ratio ofdrop
Genes on different chromosomes assortdrop
The double recessive makes up this fraction of a 9:3:3:1 F2:drop
Homologous pairs line up randomly duringdrop
A significant χ² deviation from 9:3:3:1 suggests the genes aredrop
07 · Check yourself

IB-style
multiple choice.

Single best answer, Paper 1 style. Pick one: you'll see why.

Score: 0 / 0