Biology by Bradford
Biology by Bradford · AP Biology · Unit 3.5

Cellular Respiration I: Glycolysis & Krebs

Every carbon out of glucose, and every electron it was holding — tracked from the cytosol to the matrix, before oxygen has appeared even once.

AP · U3.5CED 3.6Interactive lesson
Concept 01 · CED 3.6

Follow the carbon

Respiration looks like a wall of names. It is much simpler read as bookkeeping: six carbons go in, six carbon dioxides come out, and the energy goes somewhere you need to be able to point to.

Why in steps

Controlled release

Burning glucose in one go would release its energy as heat. Dozens of small steps let a cell capture much of it instead.

The carriers

NAD+ and FAD

They collect electrons and hydrogen at each oxidation step and deliver them to the chain. Reduced carriers are the real product here.

The surprise

Barely any ATP yet

All six carbons are gone by the end of Krebs, but only four ATP have been made. The payoff is in the next lesson.

Glucose6C2 × Pyruvate3C EACH2 × Acetyl CoA2C EACH · 2 CO2 OUTKrebs cycle4 CO2 OUT6 CARBONS IN · 6 CO2 OUT
Track the carbons and the pathway stops being a list of names. Glucose (6C) becomes two pyruvate (3C each); each loses one carbon as CO₂ entering the matrix; the remaining two-carbon acetyl groups lose both carbons during the cycle. Six in, six out, and no oxygen has been used at any point.
Diagram · interactive

Where each stage happens

Tap each part. Location matters more than usual here — it explains what can run without oxygen and what cannot.

OUTER MEMBRANEINTERMEMBRANE SPACEINNER MEMBRANE · CRISTAEMATRIXKREBS CYCLE HERECytosolGLYCOLYSIS HAPPENS HERECIRCULAR DNATAP A PART
Diagram

Two membranes, two compartments

Tap the outer membrane, intermembrane space, cristae, matrix, the cytosol panel, or the DNA.

Signature interactive · CED 3.6

The running tally

Five steps from glucose to the end of Krebs, counting every carbon and every carrier as you go. The last panel is the one to memorise.

Concept 02 · CED 3.6

Two ways to make ATP

Everything in this lesson uses the first mechanism, and it accounts for a small minority of the total yield. The second mechanism is the whole of the next lesson.

Direct

Substrate-level

An enzyme hands a phosphate from a substrate straight to ADP. Four per glucose, and no oxygen needed.

Indirect

Oxidative

Electrons from NADH power a proton pump; the gradient then drives ATP synthase. Around twenty-six ATP.

The consequence

Why oxygen matters

Without a final electron acceptor the carriers stay reduced, the chain backs up, and only the four direct ATP remain available.

SUBSTRATE-LEVELOXIDATIVE · NEXT LESSONSUBSTRATE–PADPPHOSPHATE HANDEDDIRECTLY, ENZYME TO ADP4 ATP PER GLUCOSEH+ GRADIENTATP SYNTHASEINDIRECT, VIA CHEMIOSMOSISMOST OF THE YIELD
The distinction is worth holding on to, because it explains fermentation exactly. Substrate-level phosphorylation needs nothing but an enzyme and a substrate, so it continues when oxygen runs out — which is why an anaerobic cell still gets two ATP per glucose and no more.
Concept map · interactive

How it all hangs together

Tap a node to light up its connections. The pink dashed link is the point of the whole lesson.

broken byproducesentersmakesfeedssome directcalledmade byenergy leaves as NADHGlucoseGlycolysisPyruvateLink reactionAcetyl CoAKrebs cycleATPSubstrate-levelNADH & FADH2
Concept map

Tap a node

Read down the left column: glucose, pyruvate, acetyl CoA, Krebs.

Retrieval · drag and drop

Fill the gaps

Drag a term into a gap, or tap a term and then tap a gap. Two terms belong nowhere.

cytosolmatrixpyruvateacetyl CoAsubstrate-leveldecarboxylationNADHoxaloacetate chemiosmosisphotolysis

Glycolysis takes place in the and splits glucose into two molecules of , generating ATP by phosphorylation. Pyruvate then enters the mitochondrial , where it loses a carbon as carbon dioxide — a — to form . This joins a four-carbon molecule called to begin the Krebs cycle, whose main product is the reduced carrier .

Check yourself · AP-style

Six questions

Single best answer. You get the reasoning as soon as you commit.