Biology by Bradford
Biology by Bradford · AP Biology · Unit 3.4

Photosynthesis

Light does one job: it lifts an electron. Everything else — the gradient, the ATP, the sugar — is that one push being converted into something a cell can keep.

AP · U3.4CED 3.5Interactive lesson
Concept 01 · CED 3.5

Two stages, two locations

Photosynthesis is not one reaction. It is a light-powered stage that makes ATP and NADPH, and a second stage that spends them on carbon — in a different part of the chloroplast.

Membrane

Light reactions

Water in, oxygen out, ATP and NADPH produced. Requires light and a membrane to hold a gradient across.

Stroma

Calvin cycle

CO₂ in, sugar out. Needs no light directly, but stops within minutes without the products of stage one.

The link

ATP and NADPH

Energy and reducing power. The empty carriers return, so the two stages regulate each other in both directions.

GRANUMSTROMADOUBLE MEMBRANEThylakoid membranePHOTOSYSTEMS, ETC, ATP SYNTHASEThylakoid lumenGRADIENT BUILT HEREStromaRUBISCO, ATP AND NADPH USED
The structure explains the division of labour. Thylakoid membranes are stacked into grana to pack an enormous membrane area — and area is what matters, because the light reactions need somewhere to hold a proton gradient. The stroma is simply the fluid around them, where soluble enzymes such as rubisco can work.
Diagram · interactive

How the two stages connect

Tap each part. Pay attention to the return arrow — it explains why blocking one stage stops the other.

Light reactionsTHYLAKOID MEMBRANEH2O IN · O2 OUTCalvin cycleSTROMACO2 IN · SUGAR OUTATPNADPHADP + Pi · NADP+NOT THE DARK REACTIONSTAP A PART
Diagram

Supply and demand

Tap either stage, the ATP or NADPH arrows, the return arrow, or the naming note.

Signature interactive · animated

Follow one electron

From a split water molecule to NADPH, through both photosystems. Watch where the electron loses energy — that is where the proton gradient is built.

Signature interactive · CED 3.5

Four steps of the Calvin cycle

Follow the carbon and the arithmetic together. The cycle spends most of its ATP rebuilding its own starting material.

Concept 02 · CED 3.5

What breaks when you block a step

Exams rarely ask you to recite the pathway. They ask what accumulates and what disappears when something is removed.

Method

Follow the traffic

Anything made before the block builds up. Anything made after it falls. Say which molecule and in which direction.

Watch for

Two-way dependence

Blocking the Calvin cycle also stops the light reactions, because NADP⁺ and ADP stop coming back.

Classic

The RuBP answer

Remove CO₂ and RuBP accumulates while G3P falls. Remove light and the reverse happens.

No lightATP AND NADPH STOPCalvin cycle halts within minutesNo CO2NOTHING TO FIXRuBP builds upG3P fallsRubisco blockedNO FIXATIONRuBP builds upeverything stopsTRACE THE MOLECULE THAT CANNOT BE MADE OR CANNOT BE USED
Three interventions, three predictable patterns. Note that the middle and right-hand columns produce the same accumulation for different reasons — if CO₂ cannot arrive or rubisco cannot act, RuBP piles up either way, which is why a good answer names the mechanism and not just the molecule.
Concept map · interactive

How it all hangs together

Tap a node to light up its connections. The pink dashed link is the one that mirrors respiration exactly.

excitesfeedspumps H+drivesspent inelectrons fromwaste isreduces inends atLight energyPhotosystemsElectron transportProton gradientATPCalvin cycleWaterOxygenNADPH
Concept map

Tap a node

Light energises an electron; everything else is that energy being converted, stored and spent.

Retrieval · drag and drop

Fill the gaps

Drag a term into a gap, or tap a term and then tap a gap. Two terms belong nowhere.

photolysisthylakoidproton gradientchemiosmosisNADPHstromarubiscoRuBP glycolysisdenaturation

The light reactions take place in the membrane, where water is split by to replace lost electrons. As electrons fall down the transport chain, protons are pumped into the lumen, creating a . Protons then flow back through ATP synthase, making ATP by . The final electron acceptor is NADP⁺, forming . Both products are released into the , where the enzyme fixes carbon dioxide onto the five-carbon molecule .

Check yourself · AP-style

Six questions

Single best answer. You get the reasoning as soon as you commit.