Biology by Bradford
Biology by Bradford · AP Biology · Unit 2 · CED 2.1 & 2.9 · supplementary

The Endomembrane System in Action

Follow one protein from the ribosome that builds it to the place it ends up — and watch it stay wrapped in membrane the entire way.

AP · Unit 2SupplementaryAnimated lesson
Supplementary · CED 2.1 & 2.9

One system, not six organelles

The nuclear envelope, ER, Golgi, vesicles, lysosomes and plasma membrane are usually taught as a list. They are better understood as a single production line that a protein travels down — enclosed the entire way.

The rule

Never in the cytosol

From the moment it is made, an exported protein is inside a membrane. That is what makes shipping digestive enzymes survivable.

The vehicle

Bud and fuse

Vesicles pinch off one compartment and merge with the next, carrying cargo and membrane in the same movement.

The switch

One tag, three fates

Every protein takes an identical route until the trans Golgi. A chemical label there decides where it ends up.

NucleusDNA TRANSCRIBEDMRNARough ERMADE AND FOLDEDPROTEIN IN A VESICLEGolgiMODIFIED AND TAGGEDVESICLEDestinationSORTED BY TAGSECRETEDLYSOSOMEMEMBRANEPROTEIN
The route as a flow chart. What travels between each pair of boxes is worth memorising separately from the boxes themselves: mRNA leaves the nucleus, but everything after that moves as cargo inside a vesicle.
Diagram · interactive

The components

Tap each part. Notice that two of them are destinations rather than machinery.

NUCLEUSROUGH ERSMOOTH ERGOLGIVESICLESLYSOSOMEPLASMAMEMBRANETAP A PART
Diagram

Start at the nucleus

Tap the nucleus, rough ER, smooth ER, Golgi, vesicles, lysosome or plasma membrane.

Signature interactive · animated

Follow one protein

Watch a single protein travel from ribosome to destination. Pick where it ends up, then run it again and pick somewhere else — the first seven steps are identical every time.

Concept 02 · the part people miss

The membrane is travelling too

It is easy to watch the cargo and forget the container. But the container is going somewhere as well, and it never gets thrown away.

Conserved

Handed on

Every fusion donates a patch of membrane to the receiving compartment. ER becomes vesicle, vesicle becomes Golgi, Golgi becomes cell surface.

Balanced

Taken back

Endocytosis retrieves membrane from the surface, so a secreting cell does not inflate. Traffic runs in both directions.

Topological

Inside becomes outside

The ER lumen is equivalent to the outside of the cell. Two fusions later, whatever faced the lumen faces the world.

THE MEMBRANE ITSELF IS THE CARGOER membraneVesicle wallGolgi membraneCell surfaceEVERY FUSION HANDS MEMBRANE ON · NONE IS DISCARDEDThe lumen of the ER becomes the outside of the cell after two fusions.
Follow the membrane rather than the cargo and one exam favourite falls out immediately: sugars attached to a protein inside the Golgi end up on the external surface of the cell, because the compartment turns itself inside out when the vesicle fuses.
Concept map · interactive

How it all hangs together

Tap a node to light up its connections. The pink dashed link is the principle the whole system exists to protect.

threads intobuds offdelivers toaddsdecidesalso suppliesmoved byrenewed bycargo never meets cytosolBound ribosomeRough ERVesicleGolgi complexAddress tagDestinationMembrane lipidFusionCell surface
Concept map

Tap a node

One route, one decision point, three destinations.

Retrieval · drag and drop

Fill the gaps

Drag a term into a gap, or tap a term and then tap a gap. Two terms belong nowhere.

signal sequencelumenvesiclecistransglycosylationexocytosismannose-6-phosphate hydrolysisplasmolysis

A protein enters the endomembrane system because a directs its ribosome to the rough ER, where the growing chain is threaded into the . It then leaves enclosed in a , which fuses with the face of the Golgi. Modification such as happens as the cargo advances through the stack, and sorting occurs at the face. A protein tagged with is routed to a lysosome, whereas a secreted protein leaves the cell by .

Check yourself · AP-style

Six questions

Single best answer. You get the reasoning as soon as you commit.