Biology by Bradford
Biology by Bradford · AP Biology · Unit 2 · CED 2.7

Tonicity & Water Potential

Two terms, one equation, and a sign convention that decides more marks than anything else in the unit.

AP · Unit 2CED 2.7Interactive lesson
Concept 01 · CED 2.7

Hypertonic to what?

Tonicity is a comparison, never a property. A solution is only hypertonic relative to something else — and the same solution can be hypotonic to one cell and hypertonic to another.

Relative terms

Always a comparison

Hypertonic means more solute than the other side. Used on its own the word means nothing.

The direction

Water follows solute

More solute means lower water potential, and water moves down that gradient — every time.

The wall

Why plants differ

A cellulose wall lets pressure build until uptake stops. Animal cells have no such brake, so they burst.

HYPOTONICsolution more diluteISOTONICno differenceHYPERTONICsolution more concentratedANIMALPLANTLYSEDNORMALCRENATEDTURGIDFLACCIDPLASMOLYSED
Six outcomes from two variables. Read down each column and the difference is entirely the wall: put both cells in pure water and the animal cell lyses while the plant cell simply becomes turgid and stops. Note that the plant cell is never described as bursting, and the animal cell is never described as plasmolysed.
Diagram · interactive

Inside a turgid cell

Tap each part. The two terms of the equation are both visible here — one in the vacuole, one at the wall.

CELL WALLPLASMA MEMBRANECYTOPLASMVACUOLECELL SAPTURGOR PRESSURE PUSHES OUTTURGID CELLΨp highΨs negativeTAP A PART
Diagram

Wall, membrane, vacuole

Tap the cell wall, the plasma membrane, the cytoplasm, the vacuole, the turgor arrows or the equation panel.

Signature interactive · CED 2.7

Work the numbers

Move the sliders and watch both water potentials change. The arrow always points from higher to lower — that is the only rule you need.

States · interactive

Three states of a plant cell

The same cell in three solutions. Watch what happens to the pressure term.

Concept 02 · CED 2.7

Two terms, one sign to remember

Almost every lost mark in this topic is a sign error. Solute potential is negative. Pressure potential is positive. Pure water is zero, and nothing is ever above it.

Solute term

Ψs = −iCRT

i is the number of particles per formula unit: 1 for sucrose, 2 for NaCl. Always negative.

Pressure term

Ψp

Zero in an open beaker and in a flaccid cell. Positive in a turgid one, because the wall pushes back.

The rule

Higher to lower

Water moves from higher Ψ to lower Ψ. −3 is higher than −7, and that catches people out constantly.

SOLUTION · 0.3 M SUCROSEΨs = −iCRT= −(1)(0.3)(0.0831)(295)Ψ = −7.35 barCELLΨs = −9.2 Ψp = +1.4Ψ = Ψp + ΨsΨ = −7.80 barWATER MOVES FROM −7.35 TO −7.80 · INTO THE CELL
Work each side to a single number before comparing anything. Here the solution sits at −7.35 bar and the cell at −7.80, so water enters — even though the cell has the more concentrated sap, because its pressure potential is already pushing back.
Live model · particles

Osmosis without the arrows

Water crosses freely in both directions; the solute cannot cross at all. Nothing here is being pushed.

Concept map · interactive

How it all hangs together

Tap a node to light up its connections. The pink dashed link is the one that unlocks the calculation questions.

lowerscalculated assolute termoutside cellin plantsraised bycreateslosesΨp is zero hereSolute addedWater potentialΨ = Ψp + ΨsMore negativeHypertonicPlasmolysisPressure ΨpTurgorFlaccid cell
Concept map

Tap a node

Two terms, one gradient, and a wall that changes everything for plants.

Retrieval · drag and drop

Fill the gaps

Drag a term into a gap, or tap a term and then tap a gap. Two terms belong nowhere.

water potentialhypotonichypertonicturgidplasmolysispressure potentialzeronegative denaturedsaturated

Water moves down a gradient, from the less negative side to the more negative one. A cell placed in a solution gains water, while one placed in a solution loses it. A plant cell that has gained water becomes , whereas one that has lost enough water undergoes . The wall pushing back on the protoplast is the , which is positive. In an open beaker that term is , and adding solute always makes the solute potential more .

Check yourself · AP-style

Six questions

Two of these are calculations. Single best answer, with the reasoning as soon as you commit.