IB Biology Paper 2 mock. The whole syllabus, at the real length.
A complete Paper 2 (Standard level) built from the 2025 guide: 51 marks across 9 questions, in 1 hour 30 minutes. It follows the real paper's structure and rubric below, and every question is original and tagged to the syllabus. Sit it on screen against the clock and mark yourself, print it for a class, or build a fresh one with nothing repeated.
What the real paper requires
1 hour 30 minutes · 50 marks · 44% of the final grade.
- Section A34 marks One long data question, then short-answer questions. Answer all. The opener often chains two related data sets; the short questions frequently hang several parts off one diagram.
- Section B16 marks Two extended-response questions. Answer one. Three parts from different areas of the syllabus summing to 15, plus 1 mark for how the answer is constructed.
On the front of the paper
- A calculator is required for this paper.
- Answers must be written within the answer boxes provided.
- Section B: answer one question.
How this mock is built
Section A opens with one of the longest data questions in the bank, then fills to 34 marks with short-answer questions spread evenly across the syllabus. Section B offers the real choice of two themes. Marking on screen counts only the theme you answer, plus the construction mark, so the total matches the cover.
The code on the paper rebuilds this exact mock and its markscheme. Build a fresh mock draws a new one from the same rules, so a second sitting is never the first one memorised.
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The mock
Paper code: this paper was generated, so the code is its recipe. Enter it at biologybybradford.com/exam-maker to rebuild this exact paper and its markscheme.
- Do not open this examination paper until instructed to do so.
- Section A: answer all questions.
- Section B: answer one question.
- A calculator is required for this paper.
- Answers must be written within the answer boxes provided.
Answer all questions. Answers must be written within the answer boxes provided.
Students investigated the effect of temperature on the rate of respiration of mealworm larvae (Tenebrio molitor). In experiment 1, each respirometer contained 5.0 g of larvae and a small tube of potassium hydroxide solution, which absorbs carbon dioxide. A drop of coloured liquid in a capillary tube of cross-sectional area 1.0 mm² moved towards the larvae as gas was consumed. Six respirometers were used at each temperature and the distance moved by the drop in 10 minutes was recorded.
In experiment 2, larvae were first held at 40 °C for either 10 minutes or 60 minutes and then returned to 25 °C for 30 minutes before their oxygen consumption was measured at 25 °C in the same way. A control group was kept at 25 °C throughout.
The table shows the mean distance moved by the drop in each condition with its standard error (SE).
| Experiment | Condition | Mean distance moved in 10 min / mm | ± SE / mm |
|---|---|---|---|
| 1 | 10 °C | 12 | 1.1 |
| 1 | 15 °C | 19 | 1.4 |
| 1 | 20 °C | 30 | 1.8 |
| 1 | 25 °C | 44 | 2.3 |
| 1 | 30 °C | 58 | 2.9 |
| 1 | 35 °C | 52 | 4.6 |
| 1 | 40 °C | 21 | 5.2 |
| 2 | Kept at 25 °C throughout (control) | 43 | 2.1 |
| 2 | 10 min at 40 °C, then 25 °C | 27 | 3.0 |
| 2 | 60 min at 40 °C, then 25 °C | 9 | 2.4 |
The dodo (Raphus cucullatus) of Mauritius became extinct in the late 1600s. The passenger pigeon (Ectopistes migratorius) of North America was once one of the most abundant birds on Earth, yet the last individual died in 1914.
Outline the properties of carbon atoms that allow the formation of a very large diversity of compounds in living organisms.
In an experiment, a small group of cells was taken from one region of an early-stage amphibian embryo and transplanted into a different region of a second embryo. The host cells surrounding the transplant then developed into the structures that normally form in the region from which the transplant had been taken.
The graph shows the energy changes during one reaction, catalysed by an enzyme and uncatalysed.
Outline the structure and role of transfer RNA (tRNA) in translation.
Explain why ecosystems with high biodiversity tend to be more resilient to disturbance.
Answer one question. Answers must be written within the answer boxes provided. One additional mark is available for the construction of your answers for each question.
Variation between individuals can be measured, arises from both genes and environment, and is the raw material on which selection acts.
Interactions with animals and other organisms shape how flowering plants reproduce, obtain nutrients and defend themselves.
Original practice questions © Biology by Bradford · CC BY-NC-SA 4.0 · Not affiliated with or endorsed by the International Baccalaureate Organization.
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Markscheme BbB-GQD______wAAZbns
One mark per point; / separates alternative wording within a point, OR separates alternative answers, words in brackets are not required, underlined words are essential. OWTTE = or words to that effect.
- (a) [2]
- volume of oxygen consumed = 44 (mm) × 1.0 (mm²) = 44 mm³ (in 10 minutes by 5.0 g);
- rate = 44 ÷ (5.0 × 10) = 0.88 mm³ g⁻¹ min⁻¹;
Accept 0.88 to 0.9. Award ECF for a correct method using a wrongly read distance. Units are required for the second mark.
- (b) [2 max]
- rate increases with temperature from 10 °C to 30 °C (12 mm to 58 mm);
- rate reaches a maximum at (about) 30 °C;
- above 30 °C the rate falls, steeply between 35 °C and 40 °C (52 mm to 21 mm), OWTTE;
Figures from the table are needed for at least one point.
- (c) [2]
- 25 °C versus 30 °C: significant, because the ranges of mean ± SE (41.7 to 46.3 and 55.1 to 60.9) do not overlap;
- 30 °C versus 35 °C: not significant, because the ranges of mean ± SE (55.1 to 60.9 and 47.4 to 56.6) overlap, OWTTE;
Both the conclusion and the overlap reason are required for each mark. Accept "error bars would overlap / not overlap".
- (d) [2 max]
- higher temperature gives molecules more kinetic energy, so substrates and enzymes (of cell respiration) collide more frequently;
- more of the collisions have enough energy to react / the enzyme-catalysed reactions of respiration occur faster, OWTTE;
- faster respiration uses oxygen faster (in the mitochondria / as the final electron acceptor), so the drop moves further, OWTTE;
- (e) [1]
- individual larvae differ in their tolerance of high temperature, so some are damaged / stressed more than others, giving more variable results, OWTTE; OR larvae are more active / move about at high temperature, increasing variation between respirometers, OWTTE;
Do not accept "human error" or "the equipment was less accurate" without a link to temperature.
- (f) [2 max]
- the reduction in rate caused by 40 °C is not reversed on return to 25 °C / the damage is permanent, because the treated larvae still respire more slowly than the control at 25 °C;
- longer exposure causes more damage, because 60 minutes reduced the rate (to 9 mm) more than 10 minutes (27 mm);
- the differences are significant, because the mean ± SE ranges of the control and the treated groups do not overlap, OWTTE;
Accept reference to denaturation of respiratory enzymes as a reason for the permanence.
- (g) [3 max]
- cell respiration is a (series of) chemical reactions inside cells, whereas gas exchange is the physical process of diffusion of gases across a surface;
- cell respiration releases energy from carbon compounds (glucose / fatty acids) to produce ATP, whereas gas exchange does not involve energy release / ATP production;
- cell respiration is catalysed by enzymes (in the cytoplasm and mitochondria), whereas gas exchange occurs by passive diffusion without enzymes;
- cell respiration consumes oxygen and produces carbon dioxide inside cells, whereas gas exchange moves oxygen into the organism and carbon dioxide out (across the body surface / tracheae / lungs);
- anaerobic respiration can occur without gas exchange of oxygen, whereas gas exchange of oxygen is only useful if aerobic respiration is occurring, OWTTE;
Content pivot. Award marks only for explicitly paired statements. Do not accept "respiration is breathing".
- (a) [3 max]
- in both cases humans hunted the birds directly and destroyed their habitat;
- both species were flightless or flocked predictably, making them easy to kill, and neither had evolved with human hunters;
- the dodo was also affected by introduced species (pigs, rats, monkeys) eating eggs and young, whereas the passenger pigeon was not;
- the passenger pigeon was killed on an industrial scale for markets, transported by rail and telegraph, whereas the dodo was taken by small numbers of sailors and settlers;
Award marks only for explicitly comparative statements.
- (b) [2 max]
- the species bred only in enormous colonies, so once numbers fell below a threshold breeding failed (small flocks could not raise young);
- harvesting removed adults faster than the low reproductive rate (one egg per pair) could replace them;
- loss of the mature forest that supplied its food acted at the same time as hunting, so numbers collapsed rapidly, OWTTE;
- carbon forms covalent bonds / bonds in which pairs of electrons are shared (between atoms);
- each carbon atom can form up to four bonds (with other atoms);
- the bonds can be single or double, or a combination of single and double bonds;
- carbon bonds to other carbon atoms, forming chains that can be branched or unbranched;
- carbon atoms can also form (single or multiple) rings;
- carbon bonds to atoms of other non-metallic elements (e.g. hydrogen, oxygen, nitrogen), so molecules of many shapes and sizes are possible, OWTTE;
Do not accept 'carbon forms ionic bonds'. Accept 'strong / stable bonds' as part of the first point.
- (a) [1]
- the development of an (unspecialized) cell into a specialized cell (with a particular structure and function) by expressing some genes but not others, OWTTE;
- (b) [2 max]
- the transplanted cells release a signalling chemical (that they would also have released in their original position);
- the chemical diffuses away from the transplant, forming a concentration gradient in the surrounding host tissue;
- host cells respond to the concentration they receive by switching on / expressing the genes for the structures of the donor region, OWTTE;
- the host cells had the same genome as before but their pattern of gene expression was changed by their new position in the gradient;
Accept 'morphogen' for signalling chemical. Do not accept answers based on the transplanted cells themselves forming the new structures.
- (a) [2 max]
- the enzyme lowers the activation energy (peak is lower);
- so at a given temperature more substrate molecules have enough energy to reach the transition state / react per unit time;
- binding at the active site strains/weakens bonds in the substrate, OWTTE;
- (b) [1]
- exergonic because the products are at a lower energy level than the substrates / energy is released;
- (c) [1]
- energy released depends only on the difference between substrate and product energy levels, which the enzyme does not alter / enzyme affects only the route (transition state), not the start and end points, OWTTE;
- a (small) folded RNA (cloverleaf, held by base pairing) with an anticodon of three bases;
- the (3′) end carries the specific amino acid corresponding to its anticodon (attached by a tRNA-activating enzyme);
- the anticodon pairs (complementarily) with the mRNA codon at the ribosome, delivering the correct amino acid to the growing polypeptide;
- many species can play overlapping/similar roles (redundancy);
- if one species is lost, others can (partly) take over its function;
- genetic diversity within species allows populations to adapt (to disease/change);
- complex food webs offer alternative energy pathways, so a single loss does not collapse the web, OWTTE;
- (a) D4.1.7, D4.1.8 [7 max]
- individuals vary in physical and behavioural traits, e.g. colour / size of ornaments / courtship displays / song;
- some of these traits act as signs of overall fitness / health, and are used by the opposite sex (usually females) when choosing a mate;
- individuals with the preferred traits are more successful in attracting mates / mate more often;
- (so) they produce more offspring and pass on the alleles for the trait (and for the preference);
- the trait becomes more common / more exaggerated in the population over generations;
- sexual selection can favour traits that reduce survival, e.g. conspicuous plumage, because the mating advantage outweighs the survival cost;
- example: the elaborate plumage and displays of male birds of paradise evolved through female choice;
- Endler’s guppies: in pools with few predators males became more brightly coloured / more spots, because females prefer them;
- in pools with predators, natural selection favoured duller males, which are less visible, so colour depended on the balance of the two pressures;
- (so) sexual selection and natural selection can act in opposite directions and the outcome is a compromise, OWTTE;
Accept competition between males (e.g. antlers, fighting) as sexual selection. A named example is required for full marks.
- (b) D3.2.14, D3.2.15 [4 max]
- continuous variation: the trait can take any value across a range, whereas a discrete variable such as ABO blood group falls into distinct classes;
- polygenic inheritance: several genes (at different loci) each contribute additively to the trait;
- many combinations of alleles are possible, giving many intermediate phenotypes / a normal distribution;
- environmental factors also affect the phenotype, e.g. exposure to sunlight (UV) darkens skin;
- skin colour depends on the amount of melanin, determined by several genes and by sunlight;
- data can be summarized by mean, median and mode and displayed in a box-and-whisker plot showing minimum, first quartile, median, third quartile, maximum and outliers, OWTTE;
- (c) D1.3.4, D1.3.7 [4 max]
- a gene mutation is a (random) change to the base sequence of a gene;
- mutations are caused by errors in DNA replication or DNA repair;
- (also) by mutagens: chemicals, e.g. (benzopyrene in) tobacco smoke / mustard gas, and radiation, e.g. UV / X-rays / gamma rays;
- mutations occur at random positions in the genome; no natural mechanism directs a change to a particular base to produce a desired trait;
- most mutations are harmful or neutral to the individual, and only mutations in germ cells are passed to offspring;
- (but) mutation is the original source of all genetic variation / new alleles, which is essential in the long term for evolution by natural selection, OWTTE;
Plus 1 mark for the construction of the answer: clear enough to be understood without re-reading, succinct, with little or no repetition or irrelevant material.
- (a) D3.1.8, D3.1.12 [7 max]
- male gametes are produced inside pollen grains (in the anthers), whereas female gametes / egg cells are produced inside ovules (in the ovary);
- pollination: transfer of pollen from an anther to a stigma (of the same species), by insects / wind or other agents;
- many species promote cross-pollination between different plants, increasing genetic variation;
- the pollen grain develops, growing a pollen tube down the style to the ovule;
- the male gamete (nucleus) travels down the tube and fuses with the egg cell / fertilization;
- the zygote develops into an embryo (plant) inside the ovule;
- the ovule becomes the seed, containing the embryo and food reserves, and the ovary becomes the fruit;
- seeds are dispersed away from the parent by wind / animals / water / explosive fruits, reducing competition and colonizing new areas;
- dispersal is distinct from pollination: seeds are moved rather than pollen;
- germination: the seed absorbs water, food reserves are mobilized by enzymes / hydrolysis and the embryo grows, root first then shoot;
- reproduction is sexual (meiosis and fusion of gametes) even when the plant is hermaphroditic, OWTTE;
- (b) C4.1.12 [5 max]
- mutualism is an interspecific relationship in which both species benefit;
- root nodules: (Rhizobium) bacteria in the roots of legumes / Fabaceae fix nitrogen, supplying the plant with ammonium / nitrogen compounds;
- the legume supplies the bacteria with sugars / carbon compounds from photosynthesis and a protected (low-oxygen) habitat;
- mycorrhizae: fungal hyphae associated with the roots of orchids / Orchidaceae absorb mineral ions (phosphate) and water for the plant, and supply carbon compounds to germinating orchid seeds;
- the orchid supplies the fungus with sugars / carbon compounds once it photosynthesizes;
- zooxanthellae in hard corals gain shelter, CO₂ and nitrogen compounds while supplying the coral with carbon compounds from photosynthesis / pollinators gain nectar while the plant gains pollen transfer;
- because both partners survive and reproduce better, the relationship is maintained by natural selection, OWTTE;
Accept common or scientific names. Any two of the three guide examples with benefits to both partners can reach full marks.
- (c) B4.2.9 [3 max]
- physical structures such as thorns / spines / stings / tough (silica or lignified) leaves deter or injure herbivores;
- toxic secondary compounds in leaves and seeds, e.g. alkaloids / caffeine / nicotine / cyanogenic glycosides, poison or deter herbivores;
- some herbivores have metabolic adaptations, e.g. detoxifying enzymes, that break down these toxins;
- leaf-eating insects such as caterpillars / locusts have chewing mouthparts (mandibles) to bite off and grind leaf tissue;
- other insects such as aphids have piercing mouthparts (stylets) to penetrate tissue and suck sap, OWTTE;
Plus 1 mark for the construction of the answer: clear enough to be understood without re-reading, succinct, with little or no repetition or irrelevant material.