Biology  by Bradford
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6.3 Transcription and RNA Processing: revision map

The subtopic in the middle and five tasks round it: label two diagrams, complete a table, put a process in order and fill 12 gaps. Every box rests on a learning objective of the topic in the AP Biology course and exam description. Type into the blanks and check them, or print it on A4, Letter or A3 with an answer key.

Unit 6 practice questionsThe whole Unit 6 map

Topic 6.3

Transcription and RNA Processing

AP Biology · Unit 6 Gene Expression and RegulationName

6.3.AProcessing the transcript

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6.3.ASplicing

In eukaryotes the first transcript contains , which are cut out, and exons, which are joined together. The 5' cap and poly-A tail protect the mRNA from breakdown and help it to be exported and translated. A gene can be spliced in different ways, called alternative splicing, so one gene can give several different proteins.

6.3.ASteps of transcription

Number the steps in order.

  1. In eukaryotes, the transcript is capped, spliced and given a tail.
  2. RNA polymerase binds to the promoter.
  3. The DNA is unwound and the template strand is read 3' to 5'.
  4. At the terminator the RNA and the polymerase are released.
  5. RNA nucleotides are added to the 3' end of the growing RNA, which pairs with the template.
Topic 6.3Transcription and RNA Processing

6.3.ATranscription

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6.3.AProkaryotes and eukaryotes

ProkaryoteEukaryote
Whereone compartment, with no nucleustranscription in the nucleus, translation in the
Timingtranslation can begin while transcription goes onthe mRNA must be processed and exported first
Processingnone, poly-A tail and removal of introns
Word bank 5' capcytoplasmexonintronintronsmature mRNAmRNApoly-A tailpromoterRNA polymerasetemplate strand

Answer key

Every box on the sheet with its gaps filled in, as the subtopic reads in full.

  1. LabelTranscription

    1. RNA polymerase
    2. promoter
    3. template strand
    4. mRNA

  2. LabelProcessing the transcript

    1. 5' cap
    2. exon
    3. intron
    4. poly-A tail
    5. mature mRNA

  3. OrderSteps of transcription

    1. RNA polymerase binds to the promoter.
    2. The DNA is unwound and the template strand is read 3' to 5'.
    3. RNA nucleotides are added to the 3' end of the growing RNA, which pairs with the template.
    4. At the terminator the RNA and the polymerase are released.
    5. In eukaryotes, the transcript is capped, spliced and given a tail.

  4. TableProkaryotes and eukaryotes

    ProkaryoteEukaryote
    Whereone compartment, with no nucleustranscription in the nucleus, translation in the cytoplasm
    Timingtranslation can begin while transcription goes onthe mRNA must be processed and exported first
    Processingnone5' cap, poly-A tail and removal of introns

  5. GapsSplicing

    In eukaryotes the first transcript contains introns, which are cut out, and exons, which are joined together. The 5' cap and poly-A tail protect the mRNA from breakdown and help it to be exported and translated. A gene can be spliced in different ways, called alternative splicing, so one gene can give several different proteins.